RNA Interference-Induced Innate Immunity, Off-Target Effect, or Immune Adjuvant?
نویسندگان
چکیده
منابع مشابه
RNA Interference-Induced Innate Immunity, Off-Target Effect, or Immune Adjuvant?
RNA interference (RNAi) is a natural cellular mechanism that inhibits gene expression in a sequence-specific manner. In the last decade, RNAi has become a cornerstone in basic biological systems research and drug development efforts. The RNAi-based manipulation of mammalian cells facilitates target identification and validation; assists in identifying human disease etiologies; and expedites the...
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RNA interference (RNAi) is an intracellular mechanism for post-transcriptional gene silencing that is frequently used to study gene function. RNAi is initiated by short interfering RNA (siRNA) of approximately 21 nt in length, either generated from the double-stranded RNA (dsRNA) by using the enzyme Dicer or introduced experimentally. Following association with an RNAi silencing complex, siRNA ...
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Figure 1. Small RNA molecules, miRNA and siRNA, regulate mRNA translation through RNA interference (RNAi). Depending on the level of complementarity between the small RNA molecule and mRNA, the mRNA will be silenced (complete) or translationally repressed (partial). siRNA off-targeting effects occur through partial complementarity of the siRNA with unintended mRNA targets. Since the discovery t...
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ژورنال
عنوان ژورنال: Frontiers in Immunology
سال: 2017
ISSN: 1664-3224
DOI: 10.3389/fimmu.2017.00331